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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
02/02/2022 |
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Actualizado : |
02/02/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
TORRES, D.; DELGADO-CERRONE, L.; LUNA, L.; RESQUÍN, F.; AGUILAR, A.V.; SEBENN, A.M. |
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Afiliación : |
DIEGO GABRIEL TORRES DINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LEONARDO DELGADO-CERRONE, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, 11600 Montevideo, Uruguay.; LORENA LUNA, Centro Universitario de Tacuarembó (CUT), Ruta 5 Km 386, CEP 45000, Tacuarembó, TB, Uruguay; JOSE FERNANDO RESQUIN PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANANDA VIRGINIA AGUIAR, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Centro Nacional de Pesquisa de Florestas, Estrada da Ribeira, Km 111 - Bairro Guaraituba, 83411-000, Colombo, PR, Brazil.; ALEXANDRE MAGNO SEBBENN, Instituto Florestal de São Paulo, CP 1322, São Paulo, SP, 01059-970, Brazil. |
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Título : |
The traceability of Eucalyptus clones using molecular markers. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Silvae Genetica, 2021, 70(1) 217-225. OPEN ACCESS. https://doi.org/10.2478/sg-2021-0019 |
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DOI : |
10.2478/sg-2021-0019 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published Online 25 Dec 2021.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Corresponding author: Diego Torres-Dini, E-mail: diego.torres.dini@gmail.com |
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Contenido : |
ABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (???? ) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( Ho ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters.
© 2020 Diego Torres-Dini et al., published by Sciendo MenosABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (???? ) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( Ho ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is st... Presentar Todo |
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Palabras claves : |
Clone; Clone certification; Genotyping; Identity; Multiplex; Nurseries; Traceability. |
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Asunto categoría : |
K01 Ciencias forestales - Aspectos generales |
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URL : |
https://sciendo.com/pdf/10.2478/sg-2021-0019
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Marc : |
LEADER 02660naa a2200289 a 4500
001 1062729
005 2022-02-02
008 2021 bl uuuu u00u1 u #d
024 7 $a10.2478/sg-2021-0019$2DOI
100 1 $aTORRES, D.
245 $aThe traceability of Eucalyptus clones using molecular markers.$h[electronic resource]
260 $c2021
500 $aArticle history: Published Online 25 Dec 2021. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. Corresponding author: Diego Torres-Dini, E-mail: diego.torres.dini@gmail.com
520 $aABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (???? ) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( Ho ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters. © 2020 Diego Torres-Dini et al., published by Sciendo
653 $aClone
653 $aClone certification
653 $aGenotyping
653 $aIdentity
653 $aMultiplex
653 $aNurseries
653 $aTraceability
700 1 $aDELGADO-CERRONE, L.
700 1 $aLUNA, L.
700 1 $aRESQUÍN, F.
700 1 $aAGUILAR, A.V.
700 1 $aSEBENN, A.M.
773 $tSilvae Genetica, 2021, 70(1) 217-225. OPEN ACCESS. https://doi.org/10.2478/sg-2021-0019
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
03/02/2018 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
MONTOSSI, F.; SAN JULIÁN, R.; RISSO, D.; BERRETTA, E.J.; RÍOS, M.; FRUGONI, J.C.; ZAMIT, W.; LEVRATTO, J. |
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Afiliación : |
FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO SAN JULIAN SANCHEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO FERNANDO RISSO RIET, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELBIO JOAQUIN BERRETTA CARVALLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; M. RÍOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO CESAR FRUGONI SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WILFREDO SHAMIL ZAMIT DUARTE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN CARLOS LEVRATTO CORTES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Alternativas tecnológicas para la intensificación de la producción de carne ovina en sistemas ganaderos del Basalto, II: Producción de corderos pesados |
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Complemento del título : |
OVINOS |
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Fecha de publicación : |
1998 |
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Fuente / Imprenta : |
In: BERRETTA, E.J. (Ed.). Seminario de actualización en tecnologías para basalto. INIA Tacuarembó, 3-4 diciembre 1998. Montevideo (Uruguay): INIA, 1998. |
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Páginas : |
p. 243-256 |
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Serie : |
(INIA Serie Técnica ; 102) |
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ISSN : |
1688-9266 |
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Idioma : |
Español |
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Notas : |
INIA Tacuarembó |
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Contenido : |
Los experimentos planteados en el en gorde de corderos pesados, realizados en la Unidad Experimental «Glencoe» perteneciente a INIA Tacuarembó, ubicada en el Departamento de Paysandú (latitud 32° 01'32" S, 57° 00' 39" O), en la región ganadera pales evaluar: a) el comportamiento de diferentes combinaciones de especies y variedades forrajeras para el engorde, b) el efecto de la carga animal sobre la producción individual y por superficie, c) la calidad de las canales producidas en los diferentes tratamientos y d) la viabilidad económica de las diferentes alternativas propuestas. |
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Thesagro : |
CORDERO; ENGORDE; PRODUCCION DE CARNE; SUELO BASALTICO. |
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Asunto categoría : |
--
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7980/1/ST-102-243-256.pdf
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Marc : |
LEADER 01572naa a2200301 a 4500
001 1012215
005 2018-02-03
008 1998 bl uuuu u00u1 u #d
022 $a1688-9266
100 1 $aMONTOSSI, F.
245 $aAlternativas tecnológicas para la intensificación de la producción de carne ovina en sistemas ganaderos del Basalto, II$bProducción de corderos pesados
260 $c1998
300 $ap. 243-256
490 $a(INIA Serie Técnica ; 102)
500 $aINIA Tacuarembó
520 $aLos experimentos planteados en el en gorde de corderos pesados, realizados en la Unidad Experimental «Glencoe» perteneciente a INIA Tacuarembó, ubicada en el Departamento de Paysandú (latitud 32° 01'32" S, 57° 00' 39" O), en la región ganadera pales evaluar: a) el comportamiento de diferentes combinaciones de especies y variedades forrajeras para el engorde, b) el efecto de la carga animal sobre la producción individual y por superficie, c) la calidad de las canales producidas en los diferentes tratamientos y d) la viabilidad económica de las diferentes alternativas propuestas.
650 $aCORDERO
650 $aENGORDE
650 $aPRODUCCION DE CARNE
650 $aSUELO BASALTICO
700 1 $aSAN JULIÁN, R.
700 1 $aRISSO, D.
700 1 $aBERRETTA, E.J.
700 1 $aRÍOS, M.
700 1 $aFRUGONI, J.C.
700 1 $aZAMIT, W.
700 1 $aLEVRATTO, J.
773 $tIn: BERRETTA, E.J. (Ed.). Seminario de actualización en tecnologías para basalto. INIA Tacuarembó, 3-4 diciembre 1998. Montevideo (Uruguay): INIA, 1998.
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